Radioimmunoassay of Gastric Inhibitory Polypeptide
- 1 January 1978
- journal article
- research article
- Published by SAGE Publications in Annals of Clinical Biochemistry: International Journal of Laboratory Medicine
- Vol. 15 (1-6), 172-177
- https://doi.org/10.1177/000456327801500138
Abstract
Summary A radioimmunoassay for the measurement of gastric inhibitory polypeptide (GIP) in unextracted plasma in man has been developed using a rabbit antiserum raised against porcine GIP. Porcine GIP was employed also as standard and to produce a 125I-labelled tracer. The assay was able to distinguish 110 pg/ml GIP from zero in plasma samples. Negligible cross-reactivity was demonstrated with cholecystokinin, insulin, pancreatic polypeptide, glucagon, secretin, and vasoactive intestinal polypeptide. The mean overnight fasting plasma GIP level in 28 normal subjects was 203 pg/ml (range: undetectable—420 pg/ml). Plasma GIP levels rose, within 45 minutes of eating a mixed meal, to a mean level of 1573 pg/ml.This publication has 8 references indexed in Scilit:
- Glucagon levels in normal and diabetic subjects: Use of a specific immunoabsorbent for glucagon radioimmunoassayDiabetologia, 1977
- Radioimmunoassay for Gastric Inhibitory PolypeptideGastroenterology, 1974
- A METHOD FOR PRODUCING SPECIFIC ANTISERA WITH SMALL DOSES OF IMMUNOGENJournal of Clinical Endocrinology & Metabolism, 1971
- Further purification of a polypeptide demonstrating enterogastrone activityThe Journal of Physiology, 1970
- Preparation of highly active enterogastroneCanadian Journal of Physiology and Pharmacology, 1969
- Induction of Antibodies to Porcine ACTH in Rabbits with Nonsteroidogenic Polymers of BSA and ACTHExperimental Biology and Medicine, 1968
- THE ATTRACTIONS OF PROTEINS FOR SMALL MOLECULES AND IONSAnnals of the New York Academy of Sciences, 1949
- Demonstration of the Humoral Agent in Fat Inhibition of Gastric Secretion.Experimental Biology and Medicine, 1930