QUENCHING OF TISSUES FOR FREEZE-DRYING
- 1 January 1954
- journal article
- research article
- Published by S. Karger AG in Cells Tissues Organs
- Vol. 22 (4), 331-336
- https://doi.org/10.1159/000140966
Abstract
A quick method for quenching small pieces of animal tissues for freeze-drying is described. Use is made of the very high heat conductivity of copper by first cooling two copper discs and then freezing a thin slice of tissue between the cold metal surfaces. Simple forceps with two copper discs at the end were used. Various tissues less than 1 mm thick were quenched with these forceps previously cooled to -192[degree] C in liquid air. Microscopical examination of the subsequently freeze-dried and stained specimens did not reveal any ice-crystal-artifacts even at a magnification of 2000 x.Keywords
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