Isolation of Temperature-Sensitive Mutants of Schizosaccharomyces pombe

Abstract

Seventy-one mutants of the yeast Schizosaccharomyces pombe that were able to grow on complete medium at 25 C but not at 37 C were isolated. Strains selected for further study showed: (i) single gene mutation and (ii) cell lengthening at the restrictive temperature. Preliminary characterization of 13 mutants is reported. Seven of them have a less pronounced synthesis of deoxyribonucleic acid at the restrictive temperature, and four of them seem to be affected in cell division.