Cloning of a human gene encoding the general transcription initiation factor IIB
- 1 August 1991
- journal article
- research article
- Published by Springer Nature in Nature
- Vol. 352 (6337), 689-695
- https://doi.org/10.1038/352689a0
Abstract
Transcription factor IIB (TFIIB) has a central role in transcription of class II genes. The purification of the human TFIIB protein and isolation of a complementary DNA encoding TFIIB activity is reported here. The sequence of TFIIB, which seems to be encoded by a single gene, contains a repeated motif, in addition to a motif with similarity to the prokaryotic sigma-factors. The recombinant protein expressed in bacteria substituted for all the functions attributed to the human TFIIB protein.Keywords
This publication has 39 references indexed in Scilit:
- Factors involved in specific transcription by mammalian RNA polymerase II: role of transcription factors IIA, IID, and IIB during formation of a transcription-competent complex.Molecular and Cellular Biology, 1990
- Factors involved in specific transcription by mammalian RNA polymerase II: identification of general transcription factor TFIIG.Proceedings of the National Academy of Sciences, 1990
- Highly conserved core domain and unique N terminus with presumptive regulatory motifs in a human TATA factor (TFIID)Nature, 1990
- Functional Domains and Upstream Activation Properties of Cloned Human TATA Binding ProteinScience, 1990
- Cloning of a Transcriptionally Active Human TATA Binding FactorScience, 1990
- Initiation of transcription by RNA polymerase IIBiochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1989
- Factors Involved in Specific Transcription by Mammalian RNA Polymerase IIJournal of Biological Chemistry, 1989
- Promoter specificity and modulation of RNA polymerase II transcriptionThe FASEB Journal, 1989
- Five intermediate complexes in transcription initiation by RNA polymerase IICell, 1989
- Formation of stable preinitiation complexes between eukaryotic class B transcription factors and promoter sequencesNature, 1983