Developmental expression of choline acetyltransferase mRNA inDrosophila
- 1 November 1990
- journal article
- research article
- Published by Springer Nature in Neurochemical Research
- Vol. 15 (11), 1089-1096
- https://doi.org/10.1007/bf01101709
Abstract
We have measured the steady state levels of choline acetyltransferase (ChAT, EC 2.3.1.6) mRNA during different developmental stages ofDrosophila melanogaster using a ChAT specific cRNA probe. ChAT mRNA was first detected approximately 6–7 h after oviposition, increased until the 1st–2nd larval instar, decreased into early pupal stages and increased again during late pupation, reaching a maximum in adults. Northern analysis showed a major RNA band with a Mr of 4.7 kilobases and Western analysis also showed a single major 75 kD protein band at all developmental stages. Our results support the hypothesis that a major point of regulation of ChAT expression may be at the transcriptional level.This publication has 24 references indexed in Scilit:
- Localization of Drosophila neurons that contain choline acetyltransferase messenger RNA: An in situ hybridization studyJournal of Comparative Neurology, 1989
- Immunocytochemical study of a temperature‐sensitive choline acetyltransferase mutant of Drosophila melanogasterJournal of Comparative Neurology, 1989
- Production of Polyclonal Antisera to Choline Acetyltransferase Using a Fusion Protein Produced by a cDNA Clone VictorJournal of Neurochemistry, 1988
- Isolation of biologically active ribonucleic acid from sources enriched in ribonucleaseBiochemistry, 1979
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- Muscarinic receptor in Drosophila melanogaster demonstrated by binding of [3H]quinuclidinyl benzilateFEBS Letters, 1977
- A rapid radiochemical method for the determination of choline acetyltransferaseJournal of Neurochemistry, 1975
- Ribonucleic acid isolated by cesium chloride centrifugationBiochemistry, 1974
- Differentiation of Drosophila neuroblasts to form ganglion-like clusters of neurons in vitroCell Differentiation, 1973
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970