Spontaneous Release of Soluble HL-A Antigens from Platelets During Conservation
- 1 January 1975
- journal article
- research article
- Published by Taylor & Francis in Immunological Communications
- Vol. 4 (5), 443-451
- https://doi.org/10.3109/08820137509057332
Abstract
Experiments with the aim of studying the solubilisation of HL-A antigens from blood platelets by methods which do not involve any biologically active processes (moderate, discontinuous agitation of a low concentration of platelets suspended in a saline medium, in the presence of an antiseptic; supernatants collected at frequent intervals) have shown that platelets release membrane proteins, including HL-A antigens, spontaneously. Optimal conditions for the treatment of membrane proteins have been perfected. The great stability of HL-A antigens under these conditions permits prolonged treatment. The products extracted are soluble and extremely complex. The molecular weight of the HL-A antigens is between 40,000 and 70,000.Keywords
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