Cycloheximide Is Not a Specific Inhibitor of Protein Synthesis in Vivo
Open Access
- 1 May 1975
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 55 (5), 815-821
- https://doi.org/10.1104/pp.55.5.815
Abstract
Cycloheximide is frequently presumed to inhibit specifically the cytoplasmic protein synthesis of eukaryotes. Although previous investigators have shown that it had other effects on the cells of a variety of organisms, these results were frequently presumed to be secondary effects of the inhibition of protein synthesis. This paper shows that a wide range of deleterious effects are produced by cycloheximide on a single organism, Chlamydomonas reinhardi Dangeard. If, protein synthesis is inhibited by nonpermissive conditions in temperature-sensitive mutants or with other treatments these “secondary” effects are not produced. Instead, cycloheximide appears to have two or three independent inhibitory effects on the cell. Moreover, in contrast to a number of previous investigations, these results show that protein synthesis is not required for RNA synthesis. Instead the rate of RNA synthesis is actually increased by interference with protein synthesis.Keywords
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