Polymerase chain reaction engineering
- 20 July 1997
- journal article
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 55 (2), 359-366
- https://doi.org/10.1002/(sici)1097-0290(19970720)55:2<359::aid-bit13>3.0.co;2-c
Abstract
A mathematical model for polymerase chain reaction (PCR) is developed, taking into account the three steps in this process: melting of DNA; primer annealing; and DNA synthesis (polymerization). Activity and deactivation of the polymerase enzyme as a function of temperature is incorporated in the kinetic model to get a better understanding of the amplification of DNA. Computer simulation of the model is carried out to determine the effects of various parameters, such as the cycle number, initial DNA concentration (copynumber), initial enzyme concentration, extension time, temperature ramp, and enzyme deactivation on the DNA generation. © 1997 John Wiley & Sons, Inc. Biotechnol Bioeng55: 359–366, 1997.This publication has 16 references indexed in Scilit:
- Kinetics of deactivation for thermostable DNA polymerase enzymesBiotechnology Techniques, 1996
- High Speed Polymerase Chain Reaction in Constant FlowBioscience, Biotechnology, and Biochemistry, 1994
- Recent Advances in the Polymerase Chain ReactionScience, 1991
- Amplification of Nucleic Acids by Polymerase Chain Reaction (PCR) and Other Methods and their ApplicationsCritical Reviews in Biochemistry and Molecular Biology, 1991
- DNA amplification by the polymerase chain reactionAnalytical Chemistry, 1990
- THERMOSTABLE DNA POLYMERASESPublished by Elsevier ,1990
- OPTIMIZATION OF PCRsPublished by Elsevier ,1990
- Optimization of the annealing temperature for DNA amplificationin vitro;Nucleic Acids Research, 1990
- The polymerase chain reactionImmunology Today, 1989
- [21] Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reactionMethods in Enzymology, 1987