Real-Time PCR Assay for Detection and Genotype Differentiation of Giardia lamblia in Stool Specimens

Abstract

Real-time PCR, using dual-labeled fluorescent probes targeting the β-giardin gene, was used to detect Giardia lamblia in human stool specimens and to discriminate between isolates from the two major genetic assemblages of G. lamblia infective to humans, assemblages A and B.