Effect of cytidine‐5′‐monophosphate on peptidyl transferase activity
- 15 October 1975
- journal article
- Published by Wiley in FEBS Letters
- Vol. 58 (1-2), 94-98
- https://doi.org/10.1016/0014-5793(75)80233-x
Abstract
The transfer reaction with pA-fMet as a donor substrate is strongly stimulated by CMP, whereas the transfer reaction with CpApCpCpA-acLeu as a donor substrate is inhibited by CMP. These results indicate that the donor site of peptidyl transferase contains specific binding sites for the terminal adenosine and for the cytidylic acid residue in the terminal sequence CpCpA of tRNA and that an attachment of proper nucleotides to the donor site induces a conformational change in peptidyl transferase.Keywords
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