Propagation of Chrysanthemum in Vitro. I. Multiple Plantlets from Shoot Tips and the Establishment of Tissue Cultures1,2

Abstract

Small (0.5 mm high) shoot tips of Chrysanthemum morifolium ‘Giant #4 Indianapolis White’ were grown on Murashige-Skoog medium containing various levels of kinetin, NAA, and GA₃. Formation of roots, single or multiple shoots, plantlets and friable, hard or leafy callus depended on the hormone levels used. Multiple shoots and green leafy callus were produced on medium containing 2.0 mg/l kinetin and 0.02 mg/l NAA. The leafy callus was suitable for subculture and subsequent reorganization of plantlets. Multiple shoots were rooted and grown into normal plants or were used to start new cultures which formed more multiple shoots. This technique will be useful for storage and propagation of Chrysanthemum and especially for detection and rapid multiplication of virus-free plants.