Separation and infectivity of circular and linear forms of potato spindle tuber viroid.
- 1 September 1977
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 74 (9), 3859-3863
- https://doi.org/10.1073/pnas.74.9.3859
Abstract
Potato spindle tuber viroid can be separated into 2 fractions by polyacrylamide gel electrophoresis in the presence of formamide and urea. One fraction contains predominantly circular molecules; the 2nd fraction contains almost exclusively linear molecules. The purity of the fractions was estimated by EM of formaldehyde-denatured molecules. The length distributions of denatured circular and linear molecules were determined. Both circular and linear molecules were infectious [towards tomato.].Keywords
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