Fine structure of the band 3 protein in human red cell membranes: Freeze‐fracture studies
- 1 January 1978
- journal article
- research article
- Published by Wiley in Journal of Supramolecular Structure
- Vol. 8 (3), 325-335
- https://doi.org/10.1002/jss.400080310
Abstract
The major red cell membrane protein, band 3, is a glycoprotein which extends across the membrane from the extracellular space into the cytoplasmic compartment. It is widely held that band 3 is a component of the intramembrane particles (IMP) which can be demonstrated by freeze-fracture electron microscopy. In this study, we find that the outer surface poles of the IMP can be seen by freeze-etching after they are unmasked by proteolysis under conditions which excise the surrounding sialopeptides from the membrane. The poles appear as distinctive projections, 30–50 Å in diameter, the “ES particles.” The ES particles remain associated with the outer surface of the membrane following cleavage of the band 3 polypeptide by chymotrypsin or pronase. This is consistent with previous biochemical studies which have shown that the 38,000-dalton outer surface segment of band 3 is intercalated in the lipid bilayer. A granulofibrillar component at the inner surface of the membrane is provisonally identified as the 40,000-dalton inner-surface domain of band 3.Keywords
This publication has 17 references indexed in Scilit:
- Preparation and analysis of seven major, topographically defined fragments of band 3, the predominant transmembrane polypeptide of human erythrocyte membranesBiochemistry, 1978
- Chemical Cross-Linking: Reagents and Problems in Studies of Membrane StructureAnnual Review of Biochemistry, 1977
- Proteolytic dissection of band 3, the predominant transmembrane polypeptide of the human erythrocyte membraneBiochemistry, 1976
- THE ORGANIZATION OF PROTEINS IN THE HUMAN RED BLOOD CELL MEMBRANEThe Journal of cell biology, 1974
- Preparation of Impermeable Inside-Out and Right-Side-Out Vesicles from Erythrocyte MembranesPublished by Springer Nature ,1974
- The Morphology of Adult Red CellsPublished by Elsevier ,1974
- [16] Preparation of impermeable ghosts and inside-out vesicles from human erythrocyte membranesMethods in Enzymology, 1974
- Selective solubilization of proteins from red blood cell membranes by protein perturbantsJournal of Supramolecular Structure, 1973
- Cross-linking the major proteins of the isolated erythrocyte membraneJournal of Molecular Biology, 1972
- Electrophoretic analysis of the major polypeptides of the human erythrocyte membraneBiochemistry, 1971