Effects of parathyroid hormone on H+ and NH 4 + excretion in toad urinary bladder
- 1 December 1976
- journal article
- research article
- Published by Springer Nature in The Journal of Membrane Biology
- Vol. 30 (1), 187-196
- https://doi.org/10.1007/bf01869667
Abstract
The urinary bladder ofBufo marinus excretes H+ and NH 4 + , and the H+ excretion is increased after the animal is placed in metabolic acidosis. The present study was done to determine if parathyroid hormone could stimulate the bladder to increase the excretion of H+ and/or NH 4 + . Parathyroid hormone added to the serosal solution in a final concentration of 10 μg/ml was found to increase H+ excretion by 50% above the control hemibladders, while there was no effect on NH 4 + excretion. Parathyroid hormone had no effect on H+ excretion when added to the mucosal solution. We also performed experiments utilizing theophylline and dibutyryl cyclic AMP which mimicked those of the parathyroid hormone experiments. A dose-response analysis was performed and the results indicate that 1 μg/ml of parathyroid hormone was the minimal effective dose. These results suggest that parathyroid hormone can stimulate H+ excretion in the toad urinary bladder and this effect seems to be mediated by cyclic AMP. In addition, it was found that parathyroid hormone has no effect on NH 4 + excretion.Keywords
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