Growth factors attenuate the cholinotoxic effects of ethanol during early neuroembryogenesis in the chick embryo
- 1 January 1991
- journal article
- Published by Wiley in International Journal of Developmental Neuroscience
- Vol. 9 (3), 203-213
- https://doi.org/10.1016/0736-5748(91)90041-j
Abstract
The interaction between growth factors and ethanol on cholinergic neuronal expression was studied in the chick embryo during early neuroembryogenesis using choline acetyltransferase activity as a cholinergic marker. As we have previously reported (Brodie & Vernadakis, Dev. Brain Res. 56: 223-228, 1990; Kentroti and Vernadakis, Dev. Brain Res. 56: 205-210, 1990), ethanol administration in ovo at embryonic days 1-3 produced a 30% decrease in choline acetyltransferase activity. Nerve growth factor and epidermal growth factor administration alone, at embryonic days 1-3, produced a slight increase in choline acetyltransferase activity of both brain and spinal cord when examined at embryonic day 8. Concomitant administration of either nerve growth factor or epidermal growth factor with ethanol eliminated the decrease in choline acetyltransferase activity produced by ethanol. Moreover, administration of either nerve growth factor or epidermal growth factor at embryonic days 4-7 to embryos pretreated with ethanol at days 1-3 raised choline acetyltransferase activity to a level similar to that observed in controls. Thus the growth factors reversed the ethanol-induced cholinergic insult and restored the cholinergic population to normal. These findings provide evidence of a possible role of NGF and EGF in interfering with the neurotoxic effects of ethanol during embryogenesis.Keywords
This publication has 64 references indexed in Scilit:
- Nerve growth factor promotes survival of retrogradely labeled hippocampus-projecting neurons in the rat basal forebrain in vitroDevelopmental Brain Research, 1989
- GABAergic neurons in cultures derived from three-, six- or eight-day-old chick embryo: a biochemical and immunocytochemical studyDevelopmental Brain Research, 1988
- Cholinergic neurons in cultures derived from three-, six- or eight-day-old chick embryo: a biochemical and immunocytochemical studyDevelopmental Brain Research, 1988
- Ontogeny of the nerve growth factor receptor in primary cultures of neural crest cellsDevelopmental Brain Research, 1986
- Transient expression of a neurofilament protein by replicating neuroepithelial cells of the embryonic chick brainDevelopmental Biology, 1985
- Epidermal growth factor (EGF) stimulation of cultured brain cells. II. Increased production of extracellular soluble proteinsDevelopmental Brain Research, 1983
- Epidermal growth factor (EGF) stimulation of cultured brain cells. I. Enhancement of the developmental increase in glial enzymatic activityDevelopmental Brain Research, 1983
- Nerve growth factor (NGF) stimulation of cholinergic telencephalic neurons in aggregating cell culturesDevelopmental Brain Research, 1982
- The role of nerve growth factor in vitro in cell resistance to 6-hydroxydopamine toxicityExperimental Cell Research, 1979
- Morphometric analysis of rat superior cervical ganglion after axotomy and nerve growth factor treatmentJournal of Neurocytology, 1976