Transplantation of Bone Marrow After Preservation at −70°C

Abstract

Glycerol treated adult mouse marrow was brought to −70°C at the rate of 1°C per minute to −15° and then at 10° per minute to −70°C according to the technique of Barnes and Loutit. Storage at −70°C was maintained for 8 days in one experiment and for 7 days in another. The frozen marrow was thawed by incubation at 37°C and given intravenously to adult mice after 900 r total body x-irradiation. The preserved marrow was as effective in modifying the lethal effects of irradiation as marrow obtained from the freshly killed mouse. It would seem that adult mouse bone marrow preserved by this technique can induce survival of lethally irradiated mice. Submitted on March 25, 1957