Delimitation of a promoter for RNA polymerase III by means of a functional test.

Abstract
A Xenopus laevis DNA segment containing the structural gene for .**GRAPHIC**. and 22 base pairs at the 5'' side of the gene is active in tRNA production. The DNA segment was ligated to sea urchin histone DNA and was also inserted into plasmid pCR1. Both recombinant DNA produced mature .**GRAPHIC**. at a high rate when injected into centrifuged Xenopus oocytes.