The separation of ketosteroids by partition chromatography using nitromethane as stationary phase

Publisher Website
Google Scholar
Abstract
A rapid method is descr. for the separation of some ketosteroid mixtures by partition chromatography upon columns of silicic acid containing nitromethane as stationary phase and a light petroleum-chloroform mixture saturated with nitromethane as the mobile phase. Some urinary ketosteroid extracts were fractionated in order to illustrate the utility of the method. Some observations on the production of ketosteroid artifacts during the neutral and acid extractions of urine are discussed. The method permits the separation of closely related steroid pairs such as androst-5-en-3[beta]-ol-17-one and androstan-3[beta]-ol-17-one and of androsterone (androstan-3[alpha]-ol-17-one) and testan-3[alpha]-ol-17-one.