Low Efficiency of the Moloney Murine Leukemia Virus Reverse Transcriptase during Reverse Transcription of Rare t(8;21) Fusion Gene Transcripts

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Abstract
The resolving power of RT-PCR is limited by the efficiency of RNA-to-cDNA conversion. Methods to determine this efficiency, using a real-time PCR assay for quantifying AML1-MTG 8 [t(8;21)] fusion g...