New NHR Techniques for Structure Determination and Resonance Assignments of Complex Carbohydrates

Abstract
This paper describes several new NMR techniques for structure determination and spectral assignment of polysaccharides. Positions of linkages between sugar units can be determined unambigously and with high sensitivity using a modified version of the well known INEPT experiment. A new two-dimensional experiment is shown to provide excellent resolution and sensitivity in correlating 1H and 13C chemical shifts.