No evidence for post-transcriptional control of albumin and α-fetoprotein gene expression in developing rat liver and neoplasis
Open Access
- 25 March 1982
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 10 (6), 1895-1911
- https://doi.org/10.1093/nar/10.6.1895
Abstract
Rot analysis of hybridization data using highly labeled α-fetoprotein (AFP) and albumin (P)cDNA probes has been used to quantitate AFP and albumin mRNA sequences in RNA preparations from different subcellular fractions of developing rat liver and Morris hepatoma 7777. In addition, size analysis of these mRNA sequences has been carried out by electrophoretic fractionation on agarose gels containing methylmercury hydroxyde and hybridization to radioactive cloned albumin and AFP cDNA probes. In all the tissues examined (fetal, newborn and adult rat liver, and hepatoma 7777) most of the albumin and AFP mRNA sequences were found associated with the polysomes as mature mRNA molecules ; less than 2 % of these sequences were present in the nuclear or the non polysomal cytoplasmic compartments. The number of AFP mRNA molecules was found to decrease in parallel in all the cellular compartments during rat liver development. In Morris hepatoma 7777 the content of albumin mRNA was considerably decreased in all the cellular fractions as compared to normal liver. These results demonstrate that post-tran8criptional control mechanisms leading to an accumulation of non-functional mRNA molecules are not implicated in the changes of expression of albumin and AFP genes during rat liver development and neoplasia.Keywords
This publication has 40 references indexed in Scilit:
- Relationship of rat alpha 1-fetoprotein to growth rate and chromosome composition of Morris hepatomas.1974
- Hepatocyte proliferation and alpha 1-fetoprotein in pregnant, neonatal, and partially hepatectomized rats.1974
- Effect of estrogen on gene expression in the chick oviduct. II. Transcription of chick tritiated unique deoxyribonucleic acid as measured by hybridization in ribonucleic acid excessBiochemistry, 1973
- On the lability of poly(A) sequences during extraction of messenger RNA from polyribosomesBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1972
- Early appearance of embryonic -globulin in rat serum during carcinogenesis with 4-dimethylaminoazobenzene.1971
- Antigènes cellulaires transitoires du foie de rat. I. Sécrétion et synthèse des protéines sériques fetospécifiques au cours du développement et de la régénération hépatiquesInternational Journal of Cancer, 1971
- Alpha-Fetoprotein in Ontogenesis and its Association with Malignant TumorsAdvances in Cancer Research, 1971
- The Proportion of the Incorporation of Leucine into Albumin to that into Total Protein in Rat Liver and Hepatoma Morris 5123 TC*European Journal of Biochemistry, 1969
- Sites of Serum α-Fetoprotein Synthesis in the Human and in the Rat*JCI Insight, 1967
- A procedure for the isolation of enzymically active rat-liver nucleiBiochemical Journal, 1964