DETERMINATION OF METHYLPREDNISOLONE AND HYDROCORTISONE IN PLASMA USING HIGH-PRESSURE LIQUID-CHROMATOGRAPHY

Abstract

A method for quantitative determination of methylprednisolone and hydrocortisone in plasma using added methylprednisolone acetate as internal standard and high pressure liquid chromatography is presented. Standard curves were evaluated statistically, and a lagarithmic parabolic equation is preferred over the usual standard curve mathematical relationships. The method was applied to samples of 1 subject''s [human] plasma containing methylprednisolone and endogenous hydrocortisone after oral dosing with multiple doses of methylprednisolone.