Cloning of Ly-5 cDNA.
- 1 November 1985
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 82 (21), 7360-7363
- https://doi.org/10.1073/pnas.82.21.7360
Abstract
A notable featureof Ly-5, among immunogenetic systems that identify glycoproteins of the cell surface and define the surface phenotype of cells according to their lineage, is that the Ly-5 locus specifies a range of molecular isoforms that distinguish cells of different stages and branches of hematopoietic development. The compositon of the Ly-5 locus is of much interest in regard to how these isoforms are constructed and differentially regulated according to cell lineage. We describe here a cDNA clone, pLy-5-68, that identifies Ly-5. The Ly-5 specificity of the pLy-5-68 clone was first indicated by a restriction fragment length polymorphism (RFLP), which in Southern blotting distinguishes genomic DNA of C57BL/6 (B6) mice (Ly-5a) from that of B6-Ly-5b congeneic mice whose genome is the same as B6 except for the segment of chromosome 1 that bears Ly-5b. For the following reasons it unlikely that pLy-5-68 represents a gene linked to Ly-5 allotype (Ly-5.1 vs. Ly-5.2) accorded with the RFLP pattern. Cells of the ST/bJ mouse strain have unique Ly-5 serological reactions and ST/bJ DNA gives a unique (third) RFLP pattern (Ly-5c) with pLy-5-68. All Ly-5+ cell types reacted positively with pLy-5-68 in RNA transfer blotting, and all Ly-5- cell types tested did not. The difference in size of mRNA reactive with pLy-5-68 in cells expressing the 200-kDa Ly-5 isoform as compared with cells expressing the 220-kDa Ly-5 isoform corresponded with the difference in size of the protein components of those isoforms.This publication has 15 references indexed in Scilit:
- Evidence from cDNA clones that the rat leukocyte-common antigen (T200) spans the lipid bilayer and contains a cytoplasmic domain of 80,000 MrCell, 1985
- Genomic constitution of an H-2:Tla variant leukemia.Proceedings of the National Academy of Sciences, 1984
- Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesisGene, 1983
- Hapten-reactive inducer T cells. I. Definition of two classes of hapten-specific inducer cells.The Journal of Experimental Medicine, 1983
- A cDNA cloning vector that permits expression of cDNA inserts in mammalian cells.Molecular and Cellular Biology, 1983
- Different forms of Ly-5 within the T-cell lineageImmunogenetics, 1983
- T200 cell surface glycoprotein of the mouse. Polymorphism defined by the Ly-5 system of alloantigens.The Journal of Experimental Medicine, 1980
- Preparative and analytical purification of DNA from agarose.Proceedings of the National Academy of Sciences, 1979
- DNA sequencing with chain-terminating inhibitorsProceedings of the National Academy of Sciences, 1977