Dynamic assembly of MinD on phospholipid vesicles regulated by ATP and MinE
- 30 April 2002
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 99 (10), 6761-6766
- https://doi.org/10.1073/pnas.102059099
Abstract
Selection of the division site in Escherichia coli is regulated by the min system and requires the rapid oscillation of MinD between the two halves of the cell under the control of MinE. In this study we have further investigated the molecular basis for this oscillation by examining the interaction of MinD with phospholipid vesicles. We found that MinD bound to phospholipid vesicles in the presence of ATP and, upon binding, assembled into a well-ordered helical array that deformed the vesicles into tubes. Stimulation of the MinD ATPase by addition of MinE led to disassembly of the tubes and the release of MinD from the vesicles. It is proposed that this MinE-regulated dynamic assembly of MinD underlies MinD oscillation.This publication has 44 references indexed in Scilit:
- Assembly of an FtsZ Mutant Deficient in GTPase Activity Has Implications for FtsZ Assembly and the Role of the Z Ring in Cell DivisionJournal of Bacteriology, 2001
- Dynamic Compartmentalization of Bacteria: Accurate Division inE. ColiPhysical Review Letters, 2001
- The Dimerization Function of MinC Resides in a Structurally Autonomous C-Terminal DomainJournal of Bacteriology, 2001
- Gonococcal MinD Affects Cell Division in Neisseria gonorrhoeae and Escherichia coli and Exhibits a Novel Self-InteractionJournal of Bacteriology, 2001
- FtsZ ring clusters in min and partition mutants: role of both the Min system and the nucleoid in regulating FtsZ ring localizationMolecular Microbiology, 1999
- Tubulin and FtsZ form a distinct family of GTPasesNature Structural & Molecular Biology, 1998
- SPIDER and WEB: Processing and Visualization of Images in 3D Electron Microscopy and Related FieldsJournal of Structural Biology, 1996
- GENETIC NETWORKS CONTROLLING THE INITIATION OF SPORULATION AND THE DEVELOPMENT OF GENETIC COMPETENCE IN BACILLUS SUBTILISAnnual Review of Genetics, 1995
- A division inhibitor and a topological specificity factor coded for by the minicell locus determine proper placement of the division septum in E. coliCell, 1989
- Partition of unit-copy miniplasmids to daughter cellsJournal of Molecular Biology, 1985