CONTROL OF CLONING OF NORMAL HUMAN T-LYMPHOCYTES BY TRANSFERRIN, ALBUMIN AND DIFFERENT LECTINS
- 1 January 1978
- journal article
- research article
- Vol. 33 (3), 495-498
Abstract
Normal human T[thymus derived]-lymphocytes could be induced to form colonies with a high cloning efficiency by seeding the cells directly in agar with normal human plasma and the lectins concanavalin [Con] A, phytohemagglutinin or pokeweed mitogen. The requirement for human plasma could be substituted, to different degrees with different lectins, by adding transferrin and albumin to fetal calf serum. This provides a useful system for identifying specific deficiencies in the requirements for normal T-lymphocyte colony formation.This publication has 17 references indexed in Scilit:
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