ÉTUDE SUR LES ACIDES AMINÉS LIBRES DE L'HÉMOLYMPHE DES INSECTES PAR LA MÉTHODE DE CHROMATOGRAPHIE SUR PAPIER FILTRE

Abstract

A qualitative and semiquantitative analysis of the free amino acids in the hemolymph of nine insect species was carried out with the method of paper partition chromatography. Details concerning the above method as applied to insect blood analysis are given. A discussion of the results obtained with those published in the literature is included. From 16 to 24 free amino compounds are regularly identified in insect blood, many of them in high concentration. There are qualitative and quantitative differences in amino acids between different insect species. Quantitative analyses of the free amino nitrogen of insect hemolymph are presented at the end of the paper. These quantitative determinations were carried out by means of the Van Slyke gasometric apparatus using the nitrous acid reaction.