HISTOCHEMICAL DEMONSTRATION OF β-d-GLUCURONIDASE WITH A SYNTHETIC SUBSTRATE
- 1 May 1954
- journal article
- research article
- Published by SAGE Publications in Journal of Histochemistry & Cytochemistry
- Vol. 2 (3), 209-229
- https://doi.org/10.1177/2.3.209
Abstract
The synthesis of a substrate, 6-bromo-2-naphthyl β-d-glucopyruronoside, is described, and the procedure for the histochemical demonstrations of β-d-glucuronidase activity and experiments to determine the reliability of the results of localization of enzymatic activity are given. The results in normal tissues of the rat are presented. Greatest concentration of enzymatic activity was seen in liver, spleen, epithelium of gastrointestinal tract, uterus, thyroid, and in the white matter of the nervous system.Keywords
This publication has 6 references indexed in Scilit:
- COLORIMETRIC ESTIMATION OF BETA-D-GLUCURONIDASE ACTIVITY WITH 8-BENZOYLAMINO-2-NAPHTHYL BETA-D-GLUCURONIDE1953
- THE COLORIMETRIC ESTIMATION OF BETA-D-GLUCOSIDASE1952
- A CRITICAL STUDY OF THE METHODS FOR HISTOCHEMICAL LOCALIZATION OF BETA-GLUCURONIDASES1952
- THE COLORIMETRIC ESTIMATION AND HISTOCHEMICAL DEMONSTRATION OF BETA-D-GALACTOSIDASE1952
- LOCALIZATION OF ANTIGEN IN TISSUE CELLSThe Journal of Experimental Medicine, 1951
- The histochemical localization of glucuronidaseJournal of Cellular and Comparative Physiology, 1948