Control of Nitrogenase mRNA Levels by Products of Nitrate Assimilation in the Cyanobacterium Anabaena sp. Strain PCC 7120
- 1 October 1991
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 97 (2), 825-828
- https://doi.org/10.1104/pp.97.2.825
Abstract
Nitrate inhibited nitrogenase synthesis and heterocyst development in the cyanobacterium Anabaena sp. strain PCC 7120. Inhibition of dinitrogen fixation by nitrate did not take place, however, in nitrate reductase-deficient derivatives of this strain. Hybridization of total RNA isolated from cells grown on different nitrogen sources with an internal fragment of the nifD gene showed that regulation of nitrogenase activity by nitrate is exerted through a negative control of the nitrogenase mRNA levels.Keywords
This publication has 16 references indexed in Scilit:
- Identification and characterization of hetA, a gene that acts early in the process of morphological differentiation of heterocystsJournal of Bacteriology, 1990
- Expression, nucleotide sequence and mutational analysis of two open reading frames in the nif gene region of Anabaena sp. strain PCC 7120Molecular Genetics and Genomics, 1990
- Nitrogen fixation (nif) genes of the cyanobacterium Anabaena species strain PCC 7120Journal of Biological Chemistry, 1989
- Molecular cloning and nucleotide sequence analysis of the gene coding for heterocyst ferredoxin from the cyanobacterium Anabaena sp. strain PCC 7120Molecular Genetics and Genomics, 1988
- Isolation and complementation of mutants of Anabaena sp. strain PCC 7120 unable to grow aerobically on dinitrogenJournal of Bacteriology, 1988
- Effect of nitrogenous compounds on nitrogenase gene expression in anaerobic cultures of Anabaena variabilisJournal of Bacteriology, 1988
- [12] Genetic engineering of the cyanobacterial chromosomeMethods in Enzymology, 1987
- Synthesis of nitrogenase and heterocysts by Anabaena sp. CA in the presence of high levels of ammoniaJournal of Bacteriology, 1979
- A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samplesAnalytical Biochemistry, 1978
- Effects of L-methionine-DL-sulphoximine on the assimilation of newly fixed NH3, acetylene reduction and heterocyst production in Anabaena cylindricaBiochemical and Biophysical Research Communications, 1975