Purification of Interferon from Chick Embryonic Allantoic Fluids and Fibroblast Tissue Infected with Influenza Virus

Abstract

Chick interferon from allantoic fluids of eggs infected with influenza virus and from embryo fibroblast tissue culture was purified to a specific activity of 1.6 × 10⁶ units per mg. protein; for allantoic fluid interferon this represented nearly 20,000-fold purification. Such material is thought to be still impure. Purified, but not crude, interferon was found to be strictly species specific.