Oestrogen and Human T Lymphocytes: Presence of Specific Receptors in the T‐Suppressor/Cytotoxic Subset
- 1 September 1988
- journal article
- research article
- Published by Wiley in Scandinavian Journal of Immunology
- Vol. 28 (3), 345-350
- https://doi.org/10.1111/j.1365-3083.1988.tb01459.x
Abstract
Human spleen cells were investigated for specific oestrogen binding using oestradiol-17β. High affinity, low capacity binding was observed (Kd=2.5±10−10m) and the characteristics of the oestrogen receptor identified were typical of this type of species. T lymphocyte subpopulations were prepared by negative selection by means of the monoclonal antibodies Leu 2a and 3a. Oestrogen receptors were only found in T cells of the suppressor/cytotoxic (CD8-positive) subset while helper/inducer lymphocytes (CD4-positive) showed no significant steroid binding by cytosol and nuclear assays. These results are supported by data from experiments into the effects of oestradiol-17β on immunoglobulin secretion, where it was apparent that only the suppressor/cytotoxic T-cell subpopulation was responsive to the presence of the steroid.This publication has 23 references indexed in Scilit:
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