Biochemical properties of rat protein kinase C‐η expressed in COS cells
- 9 November 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 312 (2-3), 195-199
- https://doi.org/10.1016/0014-5793(92)80934-9
Abstract
Using a PKC-ε cDNA probe a cDNA for PKC-η has been cloned from a rat lung cDNA library. When expressed in COS cells, rat PKC-η appeared as an 84 kDa protein. PKC-η expressed in COS cells. was solubilized by 1% Triton X-100 and purified away from the endogenous PKC-α by ammonium sulphate fractionation. The activity of this PKC-η preparation was characterized with respect to cofactor dependence and substrate specificity, Various PKC pseudosubstrate peptides are phosphorylated by PKC-η in a phospholipid and TPA-dependent but calcium-independent manner. The polypeptide histone IIIS is a poor substrate.Keywords
This publication has 23 references indexed in Scilit:
- Expression and characterization of protein kinase C‐δEuropean Journal of Biochemistry, 1991
- Down‐regulation of a kinase defective PKC‐αFEBS Letters, 1991
- Protein kinase CPharmacology & Therapeutics, 1991
- Proteolytic activation of protein kinase C‐ɛEuropean Journal of Biochemistry, 1990
- Unique substrate specificity and regulatory properties of PKC‐ε: a rationale for diversityFEBS Letters, 1989
- An LFA-3 cDNA encodes a phospholipid-linked membrane protein homologous to its receptor CD2Nature, 1987
- The Complete Primary Structure of Protein Kinase C—the Major Phorbol Ester ReceptorScience, 1986
- Ultrasensitive Stain for Proteins in Polyacrylamide Gels Shows Regional Variation in Cerebrospinal Fluid ProteinsScience, 1981
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970