Generation by Insulin of a Chemical Mediator That Controls Protein Phosphorylation and Dephosphorylation
- 21 December 1979
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 206 (4425), 1408-1410
- https://doi.org/10.1126/science.228395
Abstract
Deproteinized skeletal muscle extracts free of major nucleotides from control and insulin-treated rats were fractionated and assayed for inhibition of protein phosphorylation by cyclic adenosine monophosphate (AMP)-dependent and -independent protein kinases. A differential effect of insulin on a particular fraction was observed on cyclic AMP-dependent protein kinase but not on cyclic AMP-independent protein kinases. This fraction that inhibited cyclic AMP-dependent protein kinase also stimulated glycogen synthase phosphoprotein phosphatase. It is proposed that this fraction may contain a mediator substance generateed in the presence of insulin.Keywords
This publication has 11 references indexed in Scilit:
- Pyruvate Dehydrogenase Activation in Adipocyte Mitochondria by an Insulin-Generated Mediator from MuscleScience, 1979
- Autoantibodies to the insulin receptor activate glycogen synthase in rat adipocytesMolecular and Cellular Biochemistry, 1978
- Activation of glycogen synthase in rat adipocytes by insulin and glucose involves increased glucose transport and phosphorylation.Journal of Biological Chemistry, 1978
- A rapid and sensitive assay method for protein kinaseAnalytical Biochemistry, 1976
- Hormonal Regulation of Cyclic‐AMP‐Dependent Protein Kinase of Rat Diaphragm by Epinephrine and InsulinEuropean Journal of Biochemistry, 1973
- Anti-Insulin Actions of a Bovine Pituitary Diabetogenic Peptide on Glycogen SynthesisProceedings of the National Academy of Sciences, 1972
- Insulin and Glycogen SynthaseDiabetes, 1972
- The influence of insulin and epinephrine on adenosine 3′,5′-phosphate and glycogen transferase in muscleBiochimica et Biophysica Acta (BBA) - General Subjects, 1969
- A rapid filter paper assay for UDPglucose-glycogen glucosyltransferase, including an improved biosynthesis of UDP-14C-glucoseAnalytical Biochemistry, 1968
- Effects of insulin treatment on muscle 3′,5′-cyclic adenylate levels in vivo and in vitroBiochimica et Biophysica Acta (BBA) - General Subjects, 1967