Cellular location affects protein stability in Escherichia coli.
- 1 March 1982
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 79 (6), 1830-1833
- https://doi.org/10.1073/pnas.79.6.1830
Abstract
The biosynthesis of [rat] preproinsulin and proinsulin in E. coli was studied by pulse-chase experiments. When E. coli is transformed with plasmids bearing a gene consisting of a fusion of preproinsulin DNA to part or to all of the DNA encoding a bacterial signal sequence, a variety of hybrid preproinsulin molecules can be made. Molecules with largely complete hybrid signal sequences are secreted into the periplasm. Molecules with defective signal sequences, lacking the hydrophobic core, are not secreted and remain in the cytoplasm. Such molecules are degraded with a 2 min half-life, whereas the molecules transported to the periplasma are at least 10 times more stable. A full-length preproinsulin precursor appears transiently before the signal sequence is cleaved off by the bacterial signal peptidase, and a model is proposed to account for this.This publication has 19 references indexed in Scilit:
- An ‘internal” signal sequence directs secretion and processing of proinsulin in bacteriaNature, 1981
- Construction of plasmid vectors with unique PstI cloning sites in a signal sequence coding regionGene, 1980
- Secretion of beta-lactamase requires the carboxy end of the proteinCell, 1980
- A bacterial clone synthesizing proinsulin.Proceedings of the National Academy of Sciences, 1978
- The Outer Membrane Proteins of Gram-Negative Bacteria: Biosynthesis, Assembly, and FunctionsAnnual Review of Biochemistry, 1978
- Synthesis of phage M13 coat protein and its assembly into membranes in vitro.Proceedings of the National Academy of Sciences, 1978
- Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma.The Journal of cell biology, 1975
- A Possible Precursor of Immunoglobulin Light ChainsNature New Biology, 1972
- Calcium-dependent bacteriophage DNA infectionJournal of Molecular Biology, 1970
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970