Demonstration with Immunofluorescence of 19S Macroglobulins and 7S Gamma Globulins in Different Cells of the Human Spleen

Abstract

Immunohistological localization of 7S and 19S gamma globulins has been performed in the cells of normal human lymphoid tissues, with the use of specific antisera conjugated with different fluorochromes. In the red pulp of the spleen a complete differentiation has been observed and the single cells appeared to contain either 7S or 19S gamma globulins, the former being about ten times more numerous than the latter. No correlation was seen between cell morphology and the class of gamma globulins produced: 7S and 19S producing cells were equally identified amongst immature "blast" elements, lymphocytoid plasma cells and mature, classical plasma cells. In contrast with the cells of the splenic red pulp, the elements of the germinal centers of lymphoid follicles in various tissues appeared to contain mixtures of 7S and 19S gamma globulins.