Quantitative Histochemistry of the Nephron IX. Distribution and Activity of Various Enzymes in the Kidney of the Dog

Abstract

(1) The optimum conditions for the measurement of the activities of lactate dehydrogenase, malate dehydrogenase, glutamate dehydrogenase, alanine aminotransferase, aspartate aminotransferase, carbonic anhydrase, D-alanine oxidase, citrate synthase, and the glutaminases were investigated in the dog kidney. (2) The activities of these enzymes were measured in the glomeruli, proximal and distal convolutions, and medullary rays dissected from 16 (i lyophilzed sections of the dog kidney. The results were compared with the activities in homogenates of the cortex.