An inhalation chamber for administering volatile compounds to animals: performance using carbon tetrachloride

Abstract

An inhalation chamber for administering volatile compounds to laboratory animals is described. Its design is simple and its operation uncomplicated. It is an aluminum box with a heated vessel beneath the lid into which the volatile compound is injected. A fan nearby distributes the vapor and prevents layering. A freely removable lid allows rapid introduction and removal of animals. The hepatotoxic agent, carbon tetrachloride, becomes homogenously mixed with air within 2 min of its introduction. Gas samples can be removed for analyses through six sampling ports at various times, levels, and locations. The dose can be regulated with precision and the results are repeatable.