Replicating Type-C Virus Particles in Monolayer Cell Cultures of Tissues From Cattle With Lymphosarcoma

Abstract

A virion with an envelope and electron-dense nucleoid, typical of the C-type viruses associated with leukemia in other species, was identified in monolayer cell cultures established from cattle with lymphosarcoma. The virus particles were morphologically and antigenically identical to those previously described in lymphocyte suspension cultures from affected cattle. The filterability and infectivity of the virus particles for calves and lambs were demonstrated. Only limited quantities of virus particles were present in cell culture fluids, but the cultures could provide a useful source of material for large-scale virus concentration and purification.