DELAYED FLUORESCENCE IN DNA-ACRIDINE DYE COMPLEXES

Abstract
Delayed fluorescence occurs in complexes of acridine dyes with native or denatured DNA and may occur in complexes with adenosine and guanosine. The delayed fluorescence is activated by absorption of light by the DNA complexes in several respects. Relatively small concentrations of dye are needed to observe delayed fluorescence. The ratio of dye-delayed fluorescence to DNA phosphorescence is an order of magnitude higher in native DNA complexes than in denatured DNA complexes. The delayed fluorescence observed in native DNA complexes has time decays that are dependent on the concentration of the dye. The emission decays faster as the concentration of dye increases.

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