Identification of gene products of the P1 operon of Mycoplasma pneumoniae

Abstract

Gene P1 of Mycoplasma pneumoniae, which codes for a major adhesin, is flanked by two sequences with open reading frames designated QRF4 and QRF6 (Inamine et al., 1988b). In order to identify proteins translated from these ORFs, gene fusions between the N‐terminus of the RNA replicase of the Escherichia coli bacteriophage MS2 and selected regions of ORF4 and ORF6 were constructed. The corresponding fusion proteins synthesized in Escherichia coli were used to immunize mice. Antisera directed against ORF4‐related sequences did not recognize M. pneumoniae antigens in Western blot analysis, but antisera directed against ORF‐6‐derived fusion proteins reacted with two M. pneumoniae proteins of 40kDa and 90kDa. In addition, some of the antisera also recognized proteins that formed in a sodium dodecyl sulphate/polyacrylamide gel a protein ladder between 115 and 145 kDa.