Performance of a Cyclone Spore Trap

Abstract

A multipurpose, volumetric, cyclone spore trap was designed and evaluted under controlled conditions and in the field. The trap detected spores in small concentrations because of its high sampling rate of 340-450 l/min and efficient spore separating ability. For the majority of dry spores tested, retention by the inlet walls of the trap did not exceed 1.6% except with ascospores of Monilinia fructicola which were impacted in the trap volute and had to be recovered by rinsing. Losses in spore viability during operation were prevented by collecting spores directly in water, by reducing sample rate to 340 l/min, and by limiting the operation period to 30 min. The cyclone spore trap is suitable for atmospheric sampling in the field or from aircraft or automobiles and can be used in wind tunnel studies.