Isolation of α‐tubulin genes from the human malaria parasite, Plasmodium falciparum: sequence analysis of α‐tubulin
- 1 November 1989
- journal article
- research article
- Published by Wiley in Molecular Microbiology
- Vol. 3 (11), 1501-1510
- https://doi.org/10.1111/j.1365-2958.1989.tb00136.x
Abstract
As a step towards indentifying exploitable differences between host and parasite at the molecular level, we have isolated and sequenced genomic clones encompassing an entire α-tubulin gene (designated α-tubulin I) from the human malaria parasite, Plasmodium falciparum. The gene, which contains two introns, encodes a product with a predicted length of 453 amino acid residues (50.3 kD). The protein sequence shows a high degree of homology to other α-tubulins, particularly that of the coccidian parasite, Toxoplasma gondii (94%), whose gene carries introns in identical positions. Only one copy of the α-tubulin I gene itself was found, although a second gene designated α-II was also identified which is closely related but which differs at both the nucleotide and amino acid sequence levels. The α-I and β-tubulin genes were found to reside on different chromosomes.Keywords
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