Isolationsbedingungen für Zellkerne aus Hefeprotoplasten

Abstract

The structure of isolated nuclei from Saccharomyces fragilis protoplasts depends on the composition of the medium used for isolation. Buffered solutions of polymers as for instance Dextran, Ficoll and Polyvinylpyrrolidon at pH 5.5–6.5 are suitable both for carefully lysing the protoplasts and for the isolation of the nuclei. The structure of isolated nuclei does not differ markedly from nuclei in living cells. Isolated nuclei do not behave like osmometers but are swelling in buffered solution of sugars such as sucrose, mannitol and sorbitol.