Isolation and properties of brain α-actinin
- 1 October 1978
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 175 (1), 63-72
- https://doi.org/10.1042/bj1750063
Abstract
Alpha-Actinin isolated from bovine brain migrated on sodium dodecyl sulphate/polyacrylamide-gel electrophoresis like muscle alpha-actinin with an apparent mol.wt. of 100000 and cross-reacted with antibodies to muscle alpha-actinin. Brain alpha-actinin modulated actin-myosin Mg2+-activated adenosine triphosphatase activity and, when bound by polystyrene particles, was found to bind muscle actin and tropomyosin from solution. Brain alpha-actinin, in conjunction with the other components of the contractile and relaxing complex, may play a role in the release of neurotransmitters from synaptic vesicles.This publication has 28 references indexed in Scilit:
- alpha-Actinin and tropomyosin interactions with a hybrid complex of erythrocyte-actin and muscle-myosin.Journal of Biological Chemistry, 1977
- Relations of the spectrin complex of human erythrocyte membranes to the actomyosins of muscle cellBiochemistry, 1976
- Interactions of actin, myosin, and a new actin-binding protein of rabbit pulmonary macrophages. II. Role in cytoplasmic movement and phagocytosis.The Journal of cell biology, 1976
- Purification and structural analysis of myosins from brain and other non-muscle tissuesJournal of Molecular Biology, 1975
- Actin in erythrocyte ghosts and its association with spectrin. Evidence for a nonfilamentous form of these two molecules in situ.The Journal of cell biology, 1975
- Coated vesicles from pig brain: Purification and biochemical characterizationJournal of Molecular Biology, 1975
- Isolation and properties of actin, myosin, and a new actinbinding protein in rabbit alveolar macrophages.Journal of Biological Chemistry, 1975
- A solid-liquid biphasic model for characterization of properties of muscle and platelet contractile proteins.Journal of Biological Chemistry, 1975
- An improved method for the preparation of α-actinin from rabbit striated muscleBiochimica et Biophysica Acta (BBA) - Protein Structure, 1970
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951