Staphylococcus aureus protein A--antibody-mediated haemagglutination for the analysis of cross-reactivity between phenol-water extracts of Bacteroides fragilis
- 1 June 1986
- journal article
- research article
- Published by Microbiology Society in Journal of Medical Microbiology
- Vol. 21 (4), 343-347
- https://doi.org/10.1099/00222615-21-4-343
Abstract
Passive haemagglutination (PHA) and Staphylococcus aureus protein A—antibody-mediated haemagglutination (SAPA–AMHA) were used to analyse the cross-reactions of rabbit antisera against four stains of Bacteroides fragilis. There was cross-reactivity between all the strains tested but strain-specific reactions were obtained with three strains. Two to 32-fold higher antibody titres were obtained with SAPA–AMHA than with PHA. The antigen concentration required in inhibition assays was up to 32-fold higher for PHA than for SAPA–AMHA. The latter is a simple and superior alternative to PHA for such studies.This publication has 11 references indexed in Scilit:
- CROSS-REACTIVITY OF BACTEROIDES FRAGILIS O ANTIGENSActa Pathologica Microbiologica Scandinavica Section B Microbiology, 2009
- Serology of tuberculosis. 1. Standardization of passive haemagglutination test for the measurement of antibodies to Mycobacterium tuberculosisTubercle, 1983
- Capsular polysaccharides and lipopolysaccharides from two Bacteroides fragilis reference strains: chemical and immunochemical characterizationJournal of Bacteriology, 1983
- DEMONSTRATION OF HEAT‐LABILE ANTIGEN(S) OF BACTEROIDES FRAGILIS ASSOCIATED WITH NON‐HOMOGENEOUS IMMUNOFLUORESCENT STAININGActa Pathologica Microbiologica Scandinavica Series B: Microbiology, 1982
- IgG antibodies against anaerobic bacteria in blood donor sera.Journal of Clinical Pathology, 1981
- Serological identification of Bacteroides species by an enzyme-linked immunosorbent assay.Journal of Clinical Pathology, 1979
- A study of the surface and somatic antigens ofBacteroides fragilisEpidemiology and Infection, 1978
- Serological studies ofBacteroides fragilisEpidemiology and Infection, 1977
- MICROMETHODS FOR THE STUDY OF PROTEINS AND ANTIBODIES .1. PROCEDURE AND GENERAL APPLICATIONS OF HEMAGGLUTINATION AND HEMAGGLUTINATION-INHIBITION REACTIONS WITH TANNIC ACID AND PROTEIN-TREATED RED BLOOD CELLS1954
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951