A p.m.r. isotope-exchange method for studying the kinetic properties of dehydrogenases in intact cells
- 15 March 1982
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 202 (3), 573-579
- https://doi.org/10.1042/bj2020573
Abstract
A method to determine the activity of dehydrogenases in an intact-cell system is described. The method involves the use of NMR to monitor bulk isotope exchange. The approach is illustrated by application to the isotope equilibration of pyruvate and lactate as catalyzed by lactate dehydrogenase in intact [human] erythrocytes. Particular problems peculiar to bulk isotope exchange and its observation by NMR are considered.This publication has 20 references indexed in Scilit:
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