Overview of Subcellular Fractionation Procedures for the YeastSaccharomyces cerevisiae
Open Access
- 1 July 2000
- journal article
- unit
- Published by Wiley in Current Protocols in Cell Biology
- Vol. 7 (1), 3.7.1-3.7.25
- https://doi.org/10.1002/0471143030.cb0307s07
Abstract
This unit provides an overview of centrifugation-based fractionation procedures adapted for the yeast Saccharomyces cerevisiae. The goals, merits, limitations, and critical parameters of are discussed in order to facilitate the development of subcellular fractionation strategies. Topics include yeast cell lysate preparation, differential velocity centrifugation, density gradient centrifugation, and the analysis of subcellular fractions.Keywords
This publication has 55 references indexed in Scilit:
- Yeast Sites on the NetScience, 1998
- The first genome from the third domain of lifeNature, 1997
- Proteome studies of Saccharomyces cerevisiae: Identification and characterization of abundant proteinsElectrophoresis, 1997
- Signal-mediated retrieval of a membrane protein from the Golgi to the ER in yeast.The Journal of cell biology, 1994
- Reconstitution of protein translocation from solubilized yeast membranes reveals topologically distinct roles for BiP and cytosolic Hsc70.The Journal of cell biology, 1993
- end3 and end4: two mutants defective in receptor-mediated and fluid-phase endocytosis in Saccharomyces cerevisiae.The Journal of cell biology, 1993
- In vitro reconstitution of intercompartmental protein transport to the yeast vacuole.The Journal of cell biology, 1990
- Detection of an intermediate compartment involved in transport of alpha-factor from the plasma membrane to the vacuole in yeast.The Journal of cell biology, 1990
- Reconstitution of SEC gene product-dependent intercompartmental protein transportCell, 1988
- Protein Localization and Membrane Traffic in YeastAnnual Review of Cell Biology, 1985