Recombination between higher plant DNA and the Ti plasmid of Agrobacterium tumefaciens

Abstract
The Ti plasmid sequences (T-DNA) from the octopine-producing crown gall tumor A6S/2 [Nicotiana tabacum] were isolated by molecular cloning, using the bacteriophage .lambda. vector Charon 4A. Analysis of the cloned DNA segments indicates that the Ti plasmid sequences are covalently joined to plant nuclear DNA. These data demonstrate that genetic recombination between a eukaryote and a prokaryote can occur as a natural phenomenon.