The application of tetrazolium bioautography to the identification of folic acid derivatives

Abstract

Tetrazolium bioautography has been applied to the identification of folates in solutions of known concentration, and satisfactory resolution has been obtained between pteroyl-L-monoglutamic acid, calcium leucovorin, and N-5-methyltetrahydrofolic acid. The technique is suitable for the identification of folates in biological solutions, and is more specific than differential microbiological assay.