Multiple label-free biodetection and quantitative DNA-binding assays on a nanomechanical cantilever array
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- 15 July 2002
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 99 (15), 9783-9788
- https://doi.org/10.1073/pnas.152330199
Abstract
We report a microarray of cantilevers to detect multiple unlabeled biomolecules simultaneously at nanomolar concentrations within minutes. Ligand-receptor binding interactions such as DNA hybridization or protein recognition occurring on microfabricated silicon cantilevers generate nanomechanical bending, which is detected optically in situ. Differential measurements including reference cantilevers on an array of eight sensors can sequence-specifically detect unlabeled DNA targets in 80-fold excess of nonmatching DNA as a background and discriminate 3' and 5' overhangs. Our experiments suggest that the nanomechanical motion originates from predominantly steric hindrance effects and depends on the concentration of DNA molecules in solution. We show that cantilever arrays can be used to investigate the thermodynamics of biomolecular interactions mechanically, and we have found that the specificity of the reaction on a cantilever is consistent with solution data. Hence cantilever arrays permit multiple binding assays in parallel and can detect femtomoles of DNA on the cantilever at a DNA concentration in solution of 75 nM.Keywords
This publication has 19 references indexed in Scilit:
- Immobilization of Nucleic Acids at Solid Surfaces: Effect of Oligonucleotide Length on Layer AssemblyBiophysical Journal, 2000
- Quantitative Measurements and Modeling of Kinetics in Nucleic Acid Monolayer Films Using SPR SpectroscopyJournal of the American Chemical Society, 2000
- Expression profiling using cDNA microarraysNature Genetics, 1999
- Molecular interactions on microarraysNature Genetics, 1999
- Using Self-Assembly To Control the Structure of DNA Monolayers on Gold: A Neutron Reflectivity StudyJournal of the American Chemical Society, 1998
- Large-Scale Identification, Mapping, and Genotyping of Single-Nucleotide Polymorphisms in the Human GenomeScience, 1998
- Characterization of DNA Probes Immobilized on Gold SurfacesJournal of the American Chemical Society, 1997
- Quartz Crystal Microbalance Study of DNA Immobilization and Hybridization for Nucleic Acid Sensor DevelopmentAnalytical Chemistry, 1997
- Enzymatic Amplification of β-Globin Genomic Sequences and Restriction Site Analysis for Diagnosis of Sickle Cell AnemiaScience, 1985
- The tension of metallic films deposited by electrolysisProceedings of the Royal Society of London. Series A, Containing Papers of a Mathematical and Physical Character, 1909