Dominant Bovine Ovarian Follicular Cysts Express Increased Levels of Messenger RNAs for Luteinizing Hormone Receptor and 3β-Hydroxysteroid Dehydrogenase Δ4,Δ5 Isomerase Compared to Normal Dominant Follicles
Open Access
- 1 August 2001
- journal article
- Published by Oxford University Press (OUP) in Biology of Reproduction
- Vol. 65 (2), 471-476
- https://doi.org/10.1095/biolreprod65.2.471
Abstract
The objective was to compare ovarian steroids and expression of mRNAs encoding cytochrome P450 side-chain cleavage, cytochrome P450 17α-hydroxylase, cytochrome P450 aromatase, 3β-hydroxysteroid dehydrogenase Δ4,Δ5 isomerase, LH, and FSH receptors and estrogen receptor-β in ovaries of cows with dominant and nondominant ovarian follicular cysts and in normal dominant follicles. Estradiol-17β, progesterone, and androstenedione concentrations were determined in follicular fluid using specific RIAs. Dominant cysts were larger than young cysts or dominant follicles, whereas nondominant cysts were intermediate. Estradiol-17β (ng/ml) and total steroids (ng/follicle) were higher in dominant cysts than in dominant follicles. Expression of LH receptor and 3β-hydroxysteroid dehydrogenase mRNAs was higher in granulosa cells of dominant cysts than in dominant follicles. Nondominant cysts had higher follicular concentrations of progesterone, lower estradiol-17β concentrations, and lower expression of steroidogenic enzyme, gonadotropin receptor, and estrogen receptor-β mRNAs than other groups. In summary, increased expression of LH receptor and 3β-hydroxysteroid dehydrogenase mRNAs in granulosa and increased follicular estradiol-17β concentrations were associated with dominant cysts compared to dominant follicles. Study of cysts at known developmental stages is useful in identifying alterations in follicular steroidogenesis.Keywords
This publication has 40 references indexed in Scilit:
- Expression of Messenger Ribonucleic Acid (mRNA) Encoding 3β-Hydroxysteroid Dehydrogenase Δ 4,Δ 5 Isomerase (3β-HSD) during Recruitment and Selection of Bovine Ovarian Follicles: Identification of Dominant Follicles by Expression of 3β-HSD mRNA within the Granulosa Cell Layer1Biology of Reproduction, 1997
- Changes in Messenger Ribonucleic Acid Encoding Luteinizing Hormone Receptor, Cytochrome P450-Side Chain Cleavage, and Aromatase are Associated with Recruitment and Selection of Bovine Ovarian Follicles1Biology of Reproduction, 1997
- Expression of Follicle-Stimulating Hormone and Luteinizing Hormone Receptor Messenger Ribonucleic Acids in Bovine Follicles during the First Follicular Wave1Biology of Reproduction, 1995
- Expression of messenger ribonucleic acid encoding cytochrome P450 side-chain cleavage, cytochrome p450 17 alpha-hydroxylase, and cytochrome P450 aromatase in bovine follicles during the first follicular wave.Endocrinology, 1995
- Ovarian Follicular Dynamics during the Estrous Cycle in Heifers Monitored by Real-Time UItrasonograph1Biology of Reproduction, 1988
- Pattern of growth of dominant follicles during the oestrous cycle of heifersReproduction, 1988
- Estrogens Enhance the Adenosine 3′,5′-Monophosphate- Mediated Induction of Follicle-Stimulating Hormone and Luteinizing Hormone Receptors in Rat Granulosa CellsEndocrinology, 1984
- The Role of Estradiol as a Biological Amplifier of the Actions of Follicle-Stimulating Hormone: In Vitro Studies in Swine Granulosa Cells*Endocrinology, 1982
- Maturation of ovarian follicles: actions and interactions of pituitary and ovarian hormones on follicular cell differentiation.Physiological Reviews, 1980
- Ovarian Follicular Development in the Rat: Hormone Receptor Regulation by Estradiol, Follicle Stimulating Hormone and Luteinizing Hormone1Endocrinology, 1976